The Importance of Spectral Scanning and Spectral Analysis for Achieving the Optimal Assay Performance

A spectral scanning instrument is very beneficial in determining the real peak wavelengths of the spectra and developing multi-label assays. This application note describes how to gain advantages from using a spectral scanning microplate reader in assay optimization. It also shows how the reagent manufacturer’s recommendations for measurement wavelengths can differ from the optimized wavelengths and how the assay environment can affect the label’s spectral properties.