The Influence of pH on Extraction Efficiency with the Tecan AC Extraction Plate™

The AC Extraction Plate with TICE™ technology simplifies the sample preparation of biological samples. For efficient extraction from a biological sample matrix into the TICE coating, it is important that the molecule is in a neutral state. This technical note provides information on the pH-related factors that influence the distribution coefficient of a molecule between the TICE coating and the aqueous medium. It also explains how the optimal extraction pH can be determined experimentally, or be approximated by calculation.