The quantification challenges of chromatin

Chromatin fragments from host cell DNA contaminate protein-based therapeutics, viral vectors, and vaccines. Regulatory guidelines mandate stringent DNA removal, but chromatin’s protein-bound structure hinders detection by intercalating dyes like PicoGreen, leading to underestimation.

Discover how to enhance detection sensitivity and ensure more accurate quantification with an alternative approach that performs DNA fragmentation prior to measurement. Learn how this minimizes the risk of undetected carryover in downstream processing and highlights the importance of nuclease treatment in host cell–based bioprocessing.