The Use of Temperature Dependent Intrinsic Fluorescence for the Analysis of the In-Process Stability of a Protein

This poster presents a study using in-process samples of a Monoclonal Antibody from a conventional purification development. Each stage of the process is assessed using a DOE approach to map the design space and find the optimal processing conditions. In parallel, a stability assessment of the antibody at each stage is carried out using the Optim® 1000 to determine the overall manufacturability of the product.