Therapeutic Peptide Catabolite Identification using MetabolitePilot™ 2.0 Software and the TripleTOF® 6600 System

Even though the principles of pharmacokinetics and metabolism of small molecule drugs apply to peptide and protein drugs, the mechanisms of metabolism are very different. Small molecule drug metabolism occurs via the conversion of the molecule or moieties into more hydrophilic species via Phase I and Phase II processes. In this tech note the in-vitro metabolism of a model therapeutic peptide Bivalirudin was investigated to highlight the new peptide catabolism workflow features of MetabolitePilot 2.0 software.