Thermal Shift Assay Using SYPRO® Orange and qTOWER 2.2 to Detect Protein Melting Temperatures

Protein thermal shift assays are sensitive and rapid tools to examine protein thermal stability, helping to evaluate protein-ligand binding and thus to finding optimal buffer conditions or analyzing protein variations. This application note demonstrates the generation of a melting curve using the qTOWER 2.2 real-time PCR thermal cycler. This allows an easy and fast determination of protein melting temperatures.