Thyroid Hormones in Serum and Plasma Using SPE Extraction and UHPLC-MS/MS Analysis

Thyroid hormones play an important role in many biological process such as growth, development and metabolism. Concentrations of circulating thyroid hormones are routinely used to assess thyroid function and diagnose thyroid disease, for correct diagnosis it is essential that the methods for quantifying the thyroid hormones are sensitive and accurate. In this application note a method development is described to measure the free and totally thyroid hormones using a mixed-mode SPE extraction procedure and UHPLCMS/MS analysis.