Top-down Characterization of Monoclonal Antibody on an Orbitrap Fusion Lumos™ Tribrid Mass Spectrometer

This application note describes a method for mass-analysis and top-down characterisation of a monoclonal antibody, conducted on a modified Orbitrap Lumos mass spectrophotometer. LC-MS analysis was performed on the intact monoclonal antibody, the IdeS sub-units and the reduced heavy chain product. ETD HD, UVPD and CID top-down fragmentation modes were applied to obtain the optimal sequence coverage.