Total Protein Profiling Employing a Novel Protein Fraction Method Combined with Tandem Mass Tag Labelling

This application note evaluates how the accuracy of protein quantitation can be affected by fractionation at the protein level. Reproducible quantitation of cellular proteins is an important step in the analysis of cellular physiology. Protein-level fractionation in conjunction with tandem mass tag methods were used, using human gastric carcinoma cell line (MKN-45) as the example.