Total Protein Quantification by Bradford Assay using the Azure Ao Microplate Reader

This application note demonstrates the quick and easy execution of The Bradford assay using the Azure Ao Microplate Reader. The Bradford assay is a colorimetric assay where a dye is added that binds directly to proteins in the mixture causing a shift in the visible color and absorbance of the dye. Throughput of the assay can be increased using a 96-well plate format and an absorbance reader like the Azure Ao Absorbance Microplate Reader, which has the ability to take measurements and immediately process the raw data to calculate the concentration of the unknowns.