Trans-Regulation of Mouse Meiotic Recombination Hotspots by Rcr1

The application note describes a study which aims to identify any trans-acting factors that control hotspot positioning in inbred mice using the KASP™ genotyping system. It focused on crossovers in an 8-Mb segment of a 100-Mb region of mouse chromosome 1, where the longer region was heterozygous C57BL/6J (B6) x CAST/EiJ (CAST) and the remainder of the genome was either similarly heterozygous or entirely homozygous B6. Genotyping of is performed using the KASP™ genotyping system.