Transcreener® ADP2 TR-FRET Red Assay: Homogeneous ADP Detection

In this study the utility of ADP detection as a means to screen inhibitor potency using a far-red, time resolved Förster resonance energy transfer (TR-FRET) format is described. This assay uses the widely adopted TR-FRET technique to take advantage of low compound interference common to this method and owed to its time-gated nature as signal is measured after most background fluorescence has subsided. This assay has proven both sensitive and robust with Z´>0.7 at low substrate conversion levels enabling enzymology during initial velocity conditions and is an important addition to the HTS toolkit.