Transcript Profiling of the <i>Listeria monocytogenes</i> Butyrate Regulon Using Novel Library Preparation Technology to Preserve Strand Specificity

This poster describes a method utilizing next generation sequencing (NGS) technology that enables first strand cDNA to be made directly into library molecules without second strand synthesis. This cuts the library preparation time in half compared to the most used stranded mRNA kit on the market, while preserving the strand specificity of the transcripts.