Transferring RapiFluor-MS Labeled N-Glycan HILIC Separations between UPLC and HPLC

This application note demonstrates that Glycan BEH Amide Columns packed with 1.7 μm and 2.5 μm particle sizes afford scalability between RapiFluor-MS labeled glycan separations performed under UPLC and HPLC-compatible conditions. Using standard LC method transfer principles to account for differences in particle diameter (dp), column length, and column internal diameter, it is shown that comparable chromatographic profiles and relative quantitation can be achieved.