Treatment of Vivaspin® Concentrators for Improved Recovery of Low-Concentrated Protein Samples

With appropriate device size and membrane cut-off selected, Vivaspin® typically yield recoveries for the concentrated sample > 90% when the starting sample contains over 0.1 mg/ml protein of interest. The intention of the following “passivation” procedure, presented in the application note, is to improve recovery of protein samples in the nano- to microgram concentration range by pre-treating the device (membrane & plastic). This “passivation” procedure is designed for Vivaspin® ultrafiltration concentrators.