Triple Well Blot Holder as a Tool for 30-Minute Western Blotting Optimization with the SNAP i.d.^TM Protein Detection System

Optimization of antibody concentration is a key requirement for successful immunodetection with both standard methodologies as well as with the SNAP i.d. system. Antibody optimizations can now be accomplished in a fraction of the time using the SNAP i.d. system’s triple well blot holder and its 30-minute processing time to evaluate up to six different antibody concentrations in parallel; the method is described within this application note. Because all of the steps (e.g., sample prep, electrophoresis, transfer, blocking, immunodetection, and visualization) are performed together with the SNAP i.d. system, experimental variability is minimized.