Trophectoderm Biopsy of Blastocysts Using the Eppendorf TransferMan^® 4m Micromanipulators Assisted by a Laser System

This application note describes the methods used to perform trophectoderm biopsies on hatching or fully hatched blastocysts. Both methods described require practice and micromanipulation skills, however, once mastered in the mouse model these techniques can be successfully transferred to human blastocysts with excellent results.