True 3D analysis of an intracellular autophagic pathway in a 3D spheroid model

3D culture models have been used for predicting drug efficacy or toxicity. In this application note, we focus on an intracellular autophagic pathway. First, we confirmed the usefulness of the EGFP-LC3 probe for monitoring the models’ autophagic status. Then, Olympus’ true 3D analysis workflow using the FLUOVIEW™ FV3000 confocal microscope and NoviSight™ software was used to quantitatively evaluate the effect of a drug candidate called chloroquine. We demonstrated that this analysis workflow offers an alternative to profiling drug candidates using in vivo studies.