Tumor Histoids: Scalable Production of Living Human Mini‐Tumors for High Throughput Drug Screening

Tumor Histoids (TH) are living three‐dimensional human mini‐tumors consisting of stroma invaded by tumor cells. They can be produced in quantity and format suitable for high throughput screening. Stromal‐epithelial interactions occur spontaneously during TH culture and model those that activate pathways controlling tumor growth, malignant progression and response to therapies in vivo. This report describes the adaptation of the original TH production method to production in Perfecta™ hanging drop plates, as well as an experiment comparing cisplatin effect on TH, tumor cell spheroids and monolayers.