Type II Pure Water Minimizes Interference in UV-VIS Spectrophotometry

This application note outlines the importance of the quality of the water used to prepare samples, standards and reagents for ultraviolet and visible spectrophotometric techniques. The recommendation is that pure water of at least Type II quality should be used to ensure the elimination of compounds which may interfere with the absorbance measurement of the compound or element under investigation. It was found that water used for UV-VIS analysis should have a resistivity > 1 MΩ.cm, a TOC of less than 50 ppb and have been previously microfiltered.