UHPLC Method Development for Simultaneous Determination of Antihypertensive Combination Agents

This application note compares the effect of different mobile phase compositions on the chromatographic result of the separation of a mixture of one calcium channel blocker (amlodipine besylate), two thiazide diuretics (hydrochlorothiazide and chlorthalidone), and three angiotensin II receptor antagonists (azilsartan, telmisartan, valsartan). The goal of this UHPLC method development was a fast separation of potential combinations of antihypertension agents.