UHPLC Separation of Triazine Herbicides at Elevated Temperature

Temperature is a key variable in high performance liquid chromatography (HPLC), influencing solute diffusion rates, mobile phase viscosity, and solubility. The net result is that separations can be performed faster without exceeding the pressure limitations of the instrument. This application uses a porous graphitic carbon stationary phase thermostatted in a high temperature column oven to separate triazine herbicides 5 to 10 times faster than is typical with conventional HPLC. This application note also documents the performance of the high temperature liquid chromatographic method, including precision of retention time and peak area, resolution, and spike recovery from several environmental water matrices.