Ultra-fast LC/MS/MS Analysis of 25-OH Vitamin D2 and D3 from Serum

This technical note describes a sensitive, rugged and robust, high-throughput method for the analysis of 25-OH vitamin D2 and 25-OH vitamin D3 in serum. Using a Kinetex® 2.6µl C18 core-shell column, with total chromatographic cycle time of 6 minutes, this method provides rapid and effective LC/MS/MS analysis of Vitamin D levels in patient serum.