Ultra-Sensitive Host Cell Protein Detection Using CESI-MS with SWATH® Acquisition

Host cell proteins (HCPs) are undesired impurities in biologic preparation processes and can negatively affect biotherapeutic quality, potency, and safety. The integration of capillary electrophoresis (CE) and electrospray ionization (ESI) into one process (CESI) presents the possibility to improve the sensitivity of HCP quantitation through reduced ion suppression and improved ionization efficiency at ultralow nanoliter per minute flow rates. This application note demonstrates the use of CESI-MS with SWATH acquisition for the ultrasensitive detection of HCPs in a representative mAb preparation.