Ultrasensitive 3D Confocal Raman Imaging

By integrating a Raman spectrometer within a state-of-the-art confocal microscope setup, Raman imaging with a spatial resolution down to 200 nm laterally and 500 nm vertically can be achieved using visible light excitation. This application note illustrates how an image can be generated by integrating over a certain Raman line in all obtained spectra or by evaluating the various peak properties such as peak-width, min/max analysis or peak position. Due to the confocal arrangement, even depth profiling and 3-D imaging are possible if the sample is transparent.