Understanding Taylor Dispersion Analysis

Taylor Dispersion Analysis (TDA) is a microcapillary flow-based technique whereby a nanoliter-scale sample pulse is injected into the laminar flow of run buffer, which then spreads out axially due to the combined actions of convection and radial diffusion. Detection of the equilibrium concentration profile (or Taylorgram) of the dispersed sample pulse allows the molecular diffusion coefficient and hence hydrodynamic radius of solute molecules to be calculated. This application note demonstrates the use of TDA with UV-selective detection using the Malvern Viscosizer.