Uniform Isotope Labeling of Eukaryotic Proteins in Methylotrophic Yeast for High-Resolution NMR Studies

This application note demonstrates a cost-effective method using uniform ¹³C, ¹⁵N labeling of mid-size eukaryotic membrane proteins in Pichia pastoris, using ¹³C-methanol as a main carbon source. The produced samples are homogeneous, functional, stable, and yield high-resolution solid-state NMR spectra suitable for structural studies.