Use- and State-Dependent NaV 1.5 Blockers on QPatch X and In Vivo and In Vitro Assays

This application poster shows that the QPatch X in multi-hole mode can be successfully used for screening Na_V1.5 blockers. The cardiac voltage dependent sodium channel (Na_V1.5) is responsible for the upstroke and directed propagation of action potentials in the heart, and is therefore a central ion channel in safety assessment and drug discovery. For drug screening, a protocol is established for QPatch X that both tests the decay of the sodium current (30 Hz pulsetrain), and the recovery of the current from this pulse-train induced decay.