Using CLARIOstar® Fluorescence Polarization Detection to Perform hERG Predictor™ Assay

The majority of drugs associated with acquired long QT syndrome cause their effects by interacting with the potassium ion channel Kv11.1, a product of the hERG gene. The Predictor™ hERG Fluorescence Polarization Assay Kit was designed to fulfil the need to screen small molecules for the interaction with hERG and thus potential for cardiotoxicity. This application note demonstrates the performance of this assay using the CLARIOstar® microplate reader to assess the binding capability of several test compounds on a single microplate.