Using ddPCR to detect rare variant alleles in cfDNA extracted on the MagBinder Fit24 Platform

Discover how a semi-automated cfDNA extraction workflow enables highly sensitive detection of rare NRAS and EGFR mutations using ddPCR — achieving detection limits as low as 0.1% allele frequency. Extraction was achieved by employing Omega Bio-tek’s Mag-Bind^® cfDNA Kit (M3298) automated on MagBinder^® Fit²⁴ Nucleic Acid Purification System. Ideal for cancer research and liquid biopsy applications.