Using Infrared Detection Increases SNR by 3.5 Fold on Nitrocellulose Colorimetric Assay-Labeled Protein Microarrays.

The sensitivity and dynamic range of colorimetric protein assays on nitrocellulose supports requires a detection method that produces low background levels on the array. Accuracy demands objective assessment of very small spots found in higher density, highly multiplexed arrays. In this application note, the Innopsys InnoScan 710IR scanner uses infra-red detection to improve sensitivity by 3-5 fold, improve quantitative resolution between analytes and improve the multiplex capacity of the calorimetric protein assay.