Using Intrinsic Tryptophan Fluorescence to Measure Heterotrimeric G-Protein Activation

This application note describes a robust automated assay system for measuring G-protein α subunit activity. This is a universal assay to measure Gαi/o activation as modulated by a variety of inputs because the assay relies on the flexible switch region SII to change conformation depending on nucleotide state. The assay is a sensitive and high-quality means to measure G-protein activation without the use of radiolabeled nucleotides.