Using Longer Aeris™ PEPTIDE Core-Shell HPLC/UHPLC Columns for Improved Peptide Mapping

When one looks at peptide mapping applications, performance requirements are significantly different versus intact protein separations, as increased retention and selectivity are required to separate the large number of peptides generated in peptide mapping applications. Because increased resolution is a higher priority versus speed, a larger particle (3.6 μm) 100 Å HPLC/UHPLC column (Aeris PEPTIDE) was developed. It is specifically designed to improve separations of peptide and peptide mapping applications by allowing the use of longer columns at lower backpressures. In this application note, the increased resolution that the longer Aeris PEPTIDE 3.6 μm XB-C18 provides will be demonstrated.