Using SapphireAmp® Fast PCR Master Mix for Rapid Colony PCR Screening

This application note demonstrates the successful use of SapphireAmp Fast PCR Master Mix to identify plasmids containing a cloned fragment of the mouse CYP27A1 gene using a fast colony PCR screening strategy. PCR was completed in less than 60 minutes and amplification products detected to confirm the presence of the CYP27A1 insert.