Using the Azure Cielo Real-time PCR System for SARS-CoV-2 viral RNA detection

For early COVID-19 detection and diagnosis, real-time PCR instruments must be capable of measuring very small amounts of viral RNA. Researchers estimate the limit of detection (LOD), or the lowest concentration of RNA detected with > 95% confidence, can be as little as 1 copy of viral RNA per μL2. In this technical note, Azure Biosystems determine the LOD for the Azure Cielo™ using SARS-CoV-2 RNA.