Using the <i>In Situ</i> PCR Adapter

The Techne in situ PCR adapter (PRIME/ISHA) can be used to convert a 96-well Prime or PrimeG gradient thermal cycler to an in situ instrument as simply as inserting a PCR plate. The adapter accommodates up to four standard glass microscope slides. In this application note the thermal profile of the adapter surface is compared to the 96-well thermal cycler block with a view to recommending ways of optimizing PCRs to achieve the required temperature changes whilst taking into account the increased thermal mass of the adapter.