Using the Waters Forensic Toxicology Screening Application Solution with UNIFI to Determine Diuretics in Urine

Diuretics are a class of pharmaceutical compounds whose primary aim is to promote urine production. They can also be used to mask the presence of banned or illegal substances by facilitating dilution of the urine and aiding excretion. Consequently the use of diuretics is prohibited for athletes by the World Anti-Doping Agency (WADA). However, a number of diuretics only ionise in negative electrospray mode (ESI-). This application note investigates methods to further extend the Forensic Toxicology Screening Application Solution with UNIFI to include compounds that ionise in negative mode and to use the method to determine the presence of diuretics in urine, particularly at concentrations below the WADA MRPL.