Utility of Hybrid Multi-Mode Readers using Both Monochromator- and Filter-Based Detection Capabilities for the Measurement of ADP Accumulation

Multi-mode microplate readers are one of the more indispensable laboratory instruments used in life science research today. Advances in reader technology now allow for an incredible array of assays to be performed with readout based on the detection of a wide range of fluorophores, luminophores, and chromophores with spectral properties in the UV to IR. In this application note, three fluorescence-based assay technologies were used to test the capabilities of each detection system. The assays utilize Fluorescence Polarization, Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET), or Fluorescence Intensity for the measurement of ADP accumulation.