β-arrestin Recruitment for GPCR Screening

In order to detect β-arrestin recruitment by GPCR activation, DiscoveRx has developed the PathHunter luminescent β-arrestin assay. This application note compared two Hamamatsu FDSS instruments (FDSS7000 and FDSS7000EX) in 1536 format using this assay. The same experiment was conducted in 384 well format using the FDSS/µCELL. This study shows the comparison (assay window, EC50 and signal to noise ratio) between the two types of detection systems using the same assay.