Validation And Comparison Of Single-Step Flash And Dual-Spectral Luciferase Reporter Gene Assays Using The Synergy™ Line Of Microplate Readers

Here we investigate the performance of a new type of dual luciferase assay using two luciferases with spectrally resolvable light emissions in either 96- or 384-well density microplates using the Thermo Scientific Gaussia-Firefly Luciferase Dual Assay or Gaussia and Renilla Luciferase Flash Assay Kits, respectively. Assay optimization was performed and assay performance was compared across several BioTek Synergy™ Multi- Mode Microplate Readers configured with injectors using lysate containing expressed luciferase enzymes.