Validation of a Novel Tumoroid-Based Cell Culture Model to Perform 3D in vitro Cell Signaling Analyses

This application note demonstrates an in vitro HTRF® microplate assay that asseses total and phosphorylated eukaryotic translation initiation factor 4E (eIF4E) using a novel 3D cell culture system. All liquid dispensing and removal steps were performed by BioTek' MultiFlo™ FX Microplate Dispenser. BioTek’s Cytation™ 3
Cell Imaging Multi-Mode Reader detected the HTRF assay’s fluorescent signals, and also imaged the tumoroids.