Validation of an Automated Method for Library Preparation for a Next Generation Sequencing-Based Assay for Oncology

Library preparation is a critical, hands-on and time-consuming step in the NGS workflow. During library preparation, each library is prepared in an independent well of a 96-well plate, encompassing several washes and magnetic bead-binding steps. This poster presents the validation and implementation of an open liquid handling platform, the VERSATM 1100 GENE (Aurora Biomed, Vancouver, BC) for medium to high-throughput library preparation for routine utilization with the Ion AmpliSeq™ Cancer Hotspot Panel v2 (CHP2) assay on FFPE clinical specimens, including FFPE Quality Control (QC) materials.