Validation of Automated Liquid-Liquid Extraction of 25-Hydroxy Vitamin D from Whole Blood or Spiked BioCell Material Samples Using VERSA Workstation

Measurement of circulating levels of 25-hydroxy vitamin D is important in the diagnosis of intestinal mal-absorption and vitamin D deficiency or intoxication in humans. This has led to exponential growth in clinical diagnosis tests for 25-hydroxyvitamin D in blood serum. Some laboratories have developed assays using LC-MS/MS tandem mass spectrometry or HPLC. However, sample preparation to meet the requirements of these innovative methods is a cumbersome process if performed manually. To assist the sample preparation, this application note presents the VERSA Workstation for automated liquid-liquid extraction of 25-hydroxyvitamin D from blood samples.