Validation of In Vitro Assays to Measure Cytotoxicity in 3D Cell Cultures

This poster presents the results of experiments designed to improve performance of assays for 3D spheroids. The lytic capacity of assay reagents was enhanced by optimizing the detergent concentration and the parameters for physical disruption necessary to extract the desired markers. Improved protocols and reagents are described to measure ATP as a cell viability marker, caspase-3/7 activity as an apoptosis marker, glutathione as a marker of oxidative stress and HIF-1 promoter driven expression of luciferase as a hypoxia marker.