Validation of the PyroMark® Q48 Autoprep Compared with the PyroMark Q24 System for Methylation-Based Age Estimation

An age prediction method has previously been established using Pyrosequencing^® and the PyroMark Q24 platform. The method is based on determination of the methylation level of a set of markers in genomic DNA extracted from the blood of an individual. Introduction of a new version of the analytical platform, the PyroMark Q48 Autoprep System, prompted us to validate the age prediction algorithm on this new automated higher-throughput platform.