Validation of VERSA 10 Workstation for Real-Time PCR Setup Using cDNA from Two Genes of Trout Fish

A major challenge in automated PCR technology is efficiently scaling up to high-throughput PCR using microliter volumes of cDNA samples while also ensuring low cross-contamination and high accuracy and reproducibility. This scientific poster demonstrates the capabilities of the VERSA 10 PCR Setup Workstation for accurate and reproducible high throughput PCR. Validation of the VERSA 10 Workstation was successfully completed for the real-time PCR setup of cDNA from two trout genes. CV% values of less than 4% were consistently achieved using 1 μL and 2 μL volumes.