Visualization of in vivo gene editing heterogeneity at the transcript level with the BaseScope duplex RNA in situ hybridization assay

In vivo gene editing in animal models is achieved by plasmid or viral vector-based delivery of the CRISPR/Cas9 molecular machinery. In this application note from Bio-Techne, explore how the BaseScope™ RNA ISH Assay is a simple and powerful tool that complements NGS and qPCR in characterization of CRISPR-mediated gene editing. Furthermore, learn how the BaseScope assay can be utilized in gene editing applications to visualize tissue and cellular biodistribution of the gene edited transcripts, measure cell-specific Cas9 mRNA expression and guide-RNA in target tissues, and more.