Visualizing Subcellular Vesicles to Quantitate Autophagy in Neuronal Cells

Autophagy can be visualized and characterized using readily available reagents. The level of staining of autophagosomes and lysosomes in live cells provides information about the mechanism of a compound’s stimulation or inhibition of the autophagy pathway. This can be accomplished using the ImageXpress® Micro XLS System, a high content screening system for widefield fluorescence or brightfield imaging of fixed or live cells, tissues, and small organisms.