Visualizing the Architecture of Cells and Tissues

Laser scanning microscopes (LSMs) have proven extremely flexible in 3D imaging in a non-destructive manner. Most difficulties encountered in biological imaging are caused by intrinsic sample characteristics that compromise 3D imaging by limiting penetration depth and cause image distortions. Discriminating fluorescent labels from autofluorescence is another common challenge in plant and animal tissue samples. this application note describes how these problems can be overcome using Zeiss' LSM technology.