Yarra 1.8μm SEC-X150 Columns - Superior Stability under Harsh Conditions

This application note demonstrates the performance advantages of Yarra 1.8μm SEC-X150 Columns from Phenomenex for the analyses of biomolecules by aqueous size exclusion chromatography (SEC). The switching of mobile phases was used to simulate stress on the silica bed. Faster flow rates served to increase the backpressure to atypical levels and the stoppage of flow after each series of steps mimicked the completion of a series of samples. Column failure was not actually achieved after this study was completed, modeling the stable performance of Yarra SEC-X150 columns over longer lifetimes.